Coding
NphT7

Part:BBa_K3726000:Design

Designed by: Jorge Fernández Méndez   Group: iGEM21_MADRID_UCM   (2021-08-03)


CDS_NphT7


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 461
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
    Illegal NgoMIV site found at 105
    Illegal NgoMIV site found at 205
    Illegal NgoMIV site found at 511
    Illegal AgeI site found at 883
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part corresponds with the codon optimized coding sequence of NphT7 enzyme.

Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942. In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases

Source

Coding sequence of this acetoacetyl CoA synthase has been found within the genome of Streptomyces sp. CL190.

Uniprot reference: https://www.uniprot.org/uniprot/D7URV0

References

E. Okamura, T. Tomita, R. Sawa, M. Nishiyama and T. Kuzuyama, "Unprecedented acetoacetyl-coenzyme A synthesizing enzyme of the thiolase superfamily involved in the mevalonate pathway", Proceedings of the National Academy of Sciences, vol. 107, no. 25, pp. 11265-11270, 2010. Available: 10.1073/pnas.1000532107